This study advances our understanding of the function of CsLOB1 and the molecular mechanism of how Xcc causes canker symptoms via CsLOB1.Ĭ3HC4-type RING finger protein CsLOB1 Xanthomonas citrus canker susceptibility gene. Tissue expression pattern and subcellular localization analyses of the CsLOB1 gene. (A) Expression pattern of the CsLOB1 gene in different tissues of Duncan grapefruit. The endogenous housekeeping gene used was GAPDH (Cs2g14940, glyceraldehyde 3‐phosphate dehydrogenase). Each value represents the mean ± standard deviation (SD) of three replicates. YL, young leaves, 15 days after flushing ML, mature leaves, over 30 days after flushing FL, folded flowers ST, stems. ![]() All tissues in each replicate are from the same plant. 35S:EYFP‐CsLOB1 was transformed into citrus protoplast via polyethylene glycol (PEG)‐calcium‐mediated transfection. CsLOB1 localization to the nucleus was confirmed by co‐localization with Hoechst 33342. The fluorescence figures were taken 10 min after Hoechst dye addition to the protoplast at the rate of 1 : 600. The green colour indicates the fluorescence of enhanced yellow fluorescent protein (EYFP). The blue colour indicates the nuclear localization stained by Hoechst. Scale bar represents 10 μm.Įctopic CsLOB1 expression in citrus induces pustule formation. (A) Diagram of the 35S:CsLOB1‐GR plasmid used to construct CsLOB1 transgenic plants. The 35S:CsLOB1‐GR construct contains green fluoresent protein (GFP) and neomycin phosphotransferase II (NptII) to facilitate the screening of transgenic plants.
0 Comments
Leave a Reply. |
AuthorWrite something about yourself. No need to be fancy, just an overview. ArchivesCategories |